Journal: AIDS (London, England)

Article Title: Placental transfer of anti-group B Streptococcus immunoglobulin G antibody subclasses from HIV-infected and uninfected women to their uninfected infants

doi: 10.1097/QAD.0000000000000923

Figure Lengend Snippet: Geometric mean concentration (GMC) transplacental transfer ratios of total and subclass IgG1, IgG2, IgG3 and IgG4 against GBS STIa, III and V in HIV-infected and HIV-uninfected mothers to their infants [95% confidence interval (CI)].

Article Snippet: Mouse monoclonal antibodies: 4E3 antihuman IgG1 H&L, HP6014 antihuman IgG2 fd gamma, HP6050 antihuman IgG3 hinge heavy chain and mouse HP6025 antihuman IgG4 Fc (FITC) (Life Technologies, Carlsbad, California, USA) (1 : 500) in blocking buffer were added and samples incubated for 20 min at 4°C.

Techniques: Concentration Assay

Journal: Journal of Clinical & Cellular Immunology

Article Title: Identification of Thyroglobulin and its Isoforms as Target Antigens for IgG4 Thyroiditis

doi: 10.4172/2155-9899.1000568

Figure Lengend Snippet: Figure 1: Representative histopathological and immunohistochemical findings in a patient with IgG4 thyroiditis; (A) Diffuse lymphocytic infiltration and fibrosis were observed in thyroid tissue; (B) Lymphoid follicle (germinal center) formation, follicular cell degeneration and eosinophilic change of thyroid follicular epithelial cells were also observed; (C, D) IgG-positive plasma cells and (E, F) IgG4-positive plasma cells were observed in the lymphocyte-infiltrated peripheral lesion, and fibrotic area (C, E) and also highly in germinal center (D, F). Magnification: hematoxylin and eosin, HE-stained, 100 (A, B), immunostaining of IgG and IgG4, 400 (C, D, E, F); Diagnostic criteria: IgG4 thyroiditis was diagnosed according to the condition when the number of IgG4-positive plasma cells are more than 20/HPF and the ratio of IgG4-positive cells to IgG-positive cells (IgG4+/IgG+cell ratio) exceeded 30%.

Article Snippet: Immunostaining for IgG4 (mouse monoclonal, HP6025, Southern Biotech, Chicago, USA; dilution 1:400) and IgG (rabbit polyclonal, Agilent, California, USA) were performed using EnVision system (Agilent, California, USA).

Techniques: Immunohistochemical staining, Clinical Proteomics, Staining, Immunostaining, Diagnostic Assay

Journal: Journal of Clinical & Cellular Immunology

Article Title: Identification of Thyroglobulin and its Isoforms as Target Antigens for IgG4 Thyroiditis

doi: 10.4172/2155-9899.1000568

Figure Lengend Snippet: Figure 2: Identification of the target antigens recognized by serum IgG4 of IgG4 thyroiditis patients by MALDI-TOF/MS analysis; (A) Five reacting bands were able to be distinguished from other bands. The western blotting was performed using first antibody as serum of an IgG4 thyroiditis patient No.3 with high serum IgG4 level; (B) Protein scores by MALDI-TOF/ MS analysis (1a, 2a, 3a, 4a, 5a) and matched sequence were identified by MASCOT search of each band (1b, 2b, 3b, 4b, 5b). Predicted protein from five bands was thyroglobulin isoform X2. Its sequence abd matched regions of each peptide are shown in white letters on a black background; (C) Total IgG, IgG1and IgG4 antibodies against extraction of the thyroid antigens were observed in IgG4 thyroiditis patients No. 4, 5, 7 and 10; (D) The presence of IgG4 antibodies to the recombinant thyroglobulin protein (MW=37.84 kDa) were confirmed in serum of No.3 and No.7 patients (black arrow). Abbreviation: M=marker; S=sample; r-Tg=recombinant thyroglobulin.

Article Snippet: Immunostaining for IgG4 (mouse monoclonal, HP6025, Southern Biotech, Chicago, USA; dilution 1:400) and IgG (rabbit polyclonal, Agilent, California, USA) were performed using EnVision system (Agilent, California, USA).

Techniques: Western Blot, Sequencing, Extraction, Recombinant, Marker

Journal: Cell Reports Methods

Article Title: A multi-omics systems vaccinology resource to develop and test computational models of immunity

doi: 10.1016/j.crmeth.2024.100731

Figure Lengend Snippet:

Article Snippet: IgG2 mouse anti-human (PE, clone HP6025) , SouthernBiotech , 9070–09; RRID:AB_2796639.

Techniques: Clinical Proteomics, Recombinant, Luminex, Lysis, Software, Derivative Assay